Southern Blot : Principle, Protocol (steps) and Uses

Blotting is primarily used in molecular biology. It is used to identify proteins and nucleic acids for diagnostic purpose. Specifically, blotting is used for identifying biomolecules like DNA, mRNA, and protein during gene expression. DNA and RNA molecules need to undergo biochemistry analysis and they are separated using the blotting method. Picture : A helpful […]

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What is Oxidase Test ? Principle, Composition, Interpretation of Results

Oxidase test is a procedure used to determine the existence of cytochrome c oxidase enzyme. What the system does is it helps the movement of electrons between redox dye- tetramethyl-p-phenylene-diamine and the bacteria’ electron donors. It helps identify bacteria capable of producing cytochrome c oxidase; an enzyme present in the bacterial electron transport chain. It […]

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Difference between Endotoxin and Exotoxin

Many microorganisms produce toxins which play an important role in pathogenicity. Toxins are categorized into two: endotoxins and exotoxins. In this article, we are going to discuss the two types of toxins, there similarities, and differences.   Image 1: Endotoxins and exotoxins as illustrated in the images above. Picture Source: differencebetween.net   Image 2: The mechanism […]

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