{"id":70,"date":"2021-01-03T16:46:45","date_gmt":"2021-01-03T16:46:45","guid":{"rendered":"https:\/\/laboratoryinfo.com\/?p=70"},"modified":"2022-03-07T19:39:24","modified_gmt":"2022-03-07T19:39:24","slug":"periodic-acid-schiff-pas-staining-technique-for-carbohydrates","status":"publish","type":"post","link":"https:\/\/laboratoryinfo.com\/periodic-acid-schiff-pas-staining-technique-for-carbohydrates\/","title":{"rendered":"Periodic Acid Schiff (PAS) Staining Technique For Carbohydrates"},"content":{"rendered":"\n<p>Periodic Acid Schiff (PAS) staining is one of the most commonly performed special staining technique in histopathology laboratory which is used to highlight molecules with high percentage of carbohydrate content such as mucin, glycogen, fungi and basement membrane in skin.<\/p>\n\n\n<style>.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-content-wrap{padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);background-color:#ecf2f7;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-contents-title-wrap{padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-contents-title{font-weight:regular;font-style:normal;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-content-wrap .kb-table-of-content-list{font-weight:regular;font-style:normal;margin-top:var(--global-kb-spacing-sm, 1.5rem);margin-right:0px;margin-bottom:0px;margin-left:0px;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-basiccircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-basiccircle .kb-table-of-contents-icon-trigger:before, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-arrowcircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-arrowcircle .kb-table-of-contents-icon-trigger:before, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-xclosecircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-xclosecircle .kb-table-of-contents-icon-trigger:before{background-color:#ecf2f7;}<\/style>\n\n\n<h2 class=\"wp-block-heading\">Principle of PAS Staining<\/h2>\n\n\n\n<p>PAS method works by exposing the tissue to periodic acid. Periodic acid acts as oxidizing agent which oxidizes compounds having free hydroxyl group (-OH group) or amino\/alkylamine group resulting in dialdehydes. These dialdehydes when exposed to Schiff\u2019s reagent, an insoluble magenta colored complex is formed. A suitable basic stain is used as counter stain.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Preparation of staining solutions<\/h2>\n\n\n\n<p>1)&nbsp;<strong>Periodic Acid Solution :<\/strong><\/p>\n\n\n\n<ul><li>Periodic Acid : 1 gram<\/li><li>Distilled water : 100 ml<\/li><\/ul>\n\n\n\n<p>2)&nbsp;<strong>Schiff\u2019s Reagent :<\/strong><\/p>\n\n\n\n<ul><li>Fuchsin Basic : 1 gm<\/li><li>Distilled water : 100 ml<\/li><li>Sodium metabisulphite : 2 gm<\/li><li>Conc. HCl : 2 ml<\/li><li>Charcoal activated : 0.3 gm<\/li><\/ul>\n\n\n\n<blockquote class=\"wp-block-quote\"><p>Dissolve basic fuchsin in boiling water, cool at 50\u00b0C and filter. Add sodium metabisulphite and HCl. Store at dark room at room temperature overnight. Add charcoal, shake for one minute and filter<\/p><\/blockquote>\n\n\n\n<h2 class=\"wp-block-heading\">Procedure of PAS Staining<\/h2>\n\n\n\n<ol><li>Bring sections to distilled water.<\/li><li>Treat with periodic acid for 5 minutes.<\/li><li>Rinse well in distilled water.<\/li><li>Cover with Schiff\u2019s reagent for 5-15 minutes.<\/li><li>Wash in running tap water for 5-10 minutes<\/li><li>Counter&nbsp;<a href=\"https:\/\/laboratoryinfo.com\/hematoxylin-and-eosin-staining\/\" target=\"_blank\" rel=\"noreferrer noopener\">stain with Herri\u2019s hematoxylin<\/a>&nbsp;for approximately 15 seconds.<\/li><li>Differentiate (if necessary) with acid alcohol and bluing as usual.<\/li><li>Wash in tap water.<\/li><li>Rinse in increasing concentration of alcohol (70, 80, 95 and 100%)<\/li><li>Clear in xylene and mount as usual.<\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\">Result<\/h2>\n\n\n\n<p>Formation of insoluble magenta colored complex denotes positive result.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-full is-resized\"><a href=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/13058-0550x0475.jpg\"><img decoding=\"async\" src=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/13058-0550x0475.jpg\" alt=\"periodic-acid-schiff-pas-stain\" class=\"wp-image-71\" width=\"400\" srcset=\"https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/13058-0550x0475.jpg 467w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/13058-0550x0475-300x213.jpg 300w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/13058-0550x0475-20x14.jpg 20w\" sizes=\"(max-width: 467px) 100vw, 467px\" \/><\/a><\/figure><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Uses of PAS Staining<\/h2>\n\n\n\n<p>PAS stain is mainly used to highlight the molecules (structures) with high percentage of carbohydrate content such as glycogen, glycoproteins, and proteoglycans typically found in&nbsp;<a href=\"https:\/\/laboratoryinfo.com\/connective-tissue-types-functions\/\">connective tissue<\/a>, glycocalyx and basal laminae.<br>PAS staining can be used to assist in the diagnosis of several medical conditions such as:<\/p>\n\n\n\n<ul><li>Glycogen storage disease (vs. other storage disease)<\/li><li>Adenocarcinoma which often secretes mucin<\/li><li>Paget\u2019s disease of breast<\/li><li>Alveolar soft part sarcoma<\/li><li>Staining macrophages in Whipple\u2019s disease<\/li><li>Erythroleukemia, Leukemia of immature RBCs<\/li><li>Fungal infection (cell wall stain magenta)<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Additional Notes<\/h2>\n\n\n\n<blockquote class=\"wp-block-quote\"><p><strong>Differentiation<\/strong>&nbsp;is the process of removing excess dyes from tissues. It is similar to decolorizing, but infers with high degree of selectivity. Differentiation can be accomplished by: Solvents (e.g. tap water in H&amp;E staining), pH control, Mordants, Oxidizers, Other Dyes<\/p><\/blockquote>\n\n\n\n<blockquote class=\"wp-block-quote\"><p><strong>Bluing<\/strong>&nbsp;step converts the initial soluble red color (of hematoxylin) within the nucleus to an insoluble blue color. Some examples of bluing solution (alkaline pH) are: ammonia water, dilute lithium carbonate, Scott\u2019s tap water (potassium carbonate, magnesium sulphate and water<\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>Periodic Acid Schiff (PAS) staining is one of the most commonly performed special staining technique in histopathology laboratory which is used to highlight molecules with high percentage of carbohydrate content such as mucin, glycogen, fungi and basement membrane in skin. Principle of PAS Staining PAS method works by exposing the tissue to periodic acid. Periodic&#8230;<\/p>\n","protected":false},"author":4,"featured_media":71,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"rank_math_lock_modified_date":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts\/70"}],"collection":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/comments?post=70"}],"version-history":[{"count":0,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts\/70\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/media\/71"}],"wp:attachment":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/media?parent=70"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/categories?post=70"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/tags?post=70"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}