{"id":6886,"date":"2022-10-05T17:32:11","date_gmt":"2022-10-05T17:32:11","guid":{"rendered":"https:\/\/laboratoryinfo.com\/?p=6886"},"modified":"2022-10-05T17:32:14","modified_gmt":"2022-10-05T17:32:14","slug":"drabkins-solution-uses-contents-procedure","status":"publish","type":"post","link":"https:\/\/laboratoryinfo.com\/drabkins-solution-uses-contents-procedure\/","title":{"rendered":"Drabkin\u2019s Solution For Hemoglobin (Hb) Estimation"},"content":{"rendered":"\n<p>Drabkin&#8217;s solution is used to estimate the amount of hemoglobin that is present in the blood of the individual. Drabkin&#8217;s solution helps in the detection of many processes such as hemolysis in RBCs, etc.<\/p>\n\n\n<style>.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-content-wrap{padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);background-color:#ecf2f7;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-contents-title-wrap{padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-contents-title{font-weight:regular;font-style:normal;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-content-wrap .kb-table-of-content-list{font-weight:regular;font-style:normal;margin-top:var(--global-kb-spacing-sm, 1.5rem);margin-right:0px;margin-bottom:0px;margin-left:0px;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-basiccircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-basiccircle .kb-table-of-contents-icon-trigger:before, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-arrowcircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-arrowcircle .kb-table-of-contents-icon-trigger:before, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-xclosecircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-xclosecircle .kb-table-of-contents-icon-trigger:before{background-color:#ecf2f7;}<\/style>\n\n\n<h2 class=\"wp-block-heading\" id=\"drabkin-s-solution-for-hemoglobin-estimation\">Drabkin\u2019s Solution For Hemoglobin Estimation<\/h2>\n\n\n\n<p>There are kits available commercially, which makes it easy to prepare the solution. When blood passes through the analyzer, these instruments estimate its level of hemoglobin.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"sample-for-the-estimation-of-hemoglobin\">Sample For The Estimation Of Hemoglobin<\/h3>\n\n\n\n<p>A sample of blood is required for the estimation of hemoglobin.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"manual-method-for-the-preparation-of-drabkin-s-solution\">Manual Method For The Preparation Of Drabkin&#8217;s Solution<\/h2>\n\n\n\n<p>For the preparation of Drabkin\u2019s solution, follow the steps below.<\/p>\n\n\n\n<ol><li>Put in one vial of Drabkin\u2019s reagent to 1000 ml of water.<\/li><li>Add 0.5 ml of 30% of Brij L23 solution, catalog number B4184, with the mixture of drabkin\u2019s reagent and water.<\/li><li>Mix well and if insoluble particles are remaining, filter those as well.<\/li><\/ol>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" width=\"600\" height=\"462\" src=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/shows-the-different-constituents-and-their-quantity-required-in-the-preparation-of-Drabkins-solution..jpg\" alt=\"shows the different constituents and their quantity required in the preparation of Drabkin's solution.\" class=\"wp-image-6985\" srcset=\"https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/shows-the-different-constituents-and-their-quantity-required-in-the-preparation-of-Drabkins-solution..jpg 600w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/shows-the-different-constituents-and-their-quantity-required-in-the-preparation-of-Drabkins-solution.-300x231.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><figcaption> <em><strong>Figure<\/strong>: shows the different constituents and their quantity required in the preparation of Drabkin&#8217;s solution.<\/em> <\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"precautions-for-drabkin-s-solution\">Precautions For Drabkin\u2019s Solution<\/h2>\n\n\n\n<ol><li>Avoid contact with eyes and skin.<\/li><li>Make sure that you don\u2019t breathe in the vapors.<\/li><li>Good ventilation is needed to prevent vapors from forming.<\/li><li>Don\u2019t eat, drink, or smoke while using this product.<\/li><li>In case, the solution becomes cloudy after the addition of blood, centrifuge before taking the readings. This occurs because of the non-hemolyzed RBCs or globulins.<\/li><li>Clean the spectrophotometer cells so that they would be free of fingerprints, or else the reading will be high<\/li><\/ol>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" width=\"500\" height=\"323\" src=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Figure-shows-the-backside-of-Drabkins-reagent-packaging-that-includes-all-the-warnings.jpg\" alt=\"Figure shows the backside of Drabkins reagent packaging that includes all the warnings\" class=\"wp-image-6983\" srcset=\"https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Figure-shows-the-backside-of-Drabkins-reagent-packaging-that-includes-all-the-warnings.jpg 500w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Figure-shows-the-backside-of-Drabkins-reagent-packaging-that-includes-all-the-warnings-300x194.jpg 300w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><figcaption> <em><strong>Figure<\/strong> : shows the backside of Drabkin&#8217;s reagent packaging that includes all the warnings.<\/em> <\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"principle-of-drabkin-s-solution\">Principle Of Drabkin\u2019s Solution<\/h2>\n\n\n\n<p>Drabkin\u2019s procedure is based on the oxidation of hemoglobin and its derivatives. However, sulfhemoglobin is not oxidized in the Drabkin\u2019s solution. Sulfhemoglobin is a pigment that occurs in the blood. It forms methemoglobin in the presence of potassium ferricyanide.<\/p>\n\n\n\n<p>The reaction of methemoglobin and potassium cyanide forms a stable product, cyanmethemoglobin. This complex compound has a maximum absorption of 540nm.&nbsp;<\/p>\n\n\n\n<blockquote class=\"wp-block-quote\"><p>Methemoglobin + potassium cyanide = cyanmethemoglobin.<\/p><\/blockquote>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img decoding=\"async\" src=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Figure-showing-the-basic-principle-of-Drabkin-s-solution-for-the-estimation-of-Hb-image.jpg\" alt=\"Figure showing the basic principle of Drabkin s solution for the estimation of Hb image\" class=\"wp-image-6981\" width=\"500\" height=\"264\" srcset=\"https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Figure-showing-the-basic-principle-of-Drabkin-s-solution-for-the-estimation-of-Hb-image.jpg 500w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Figure-showing-the-basic-principle-of-Drabkin-s-solution-for-the-estimation-of-Hb-image-300x158.jpg 300w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><figcaption> <em>Figure: showing the basic principle of Drabkin&#8217;s solution for the estimation of Hb.<\/em> <\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"procedure-to-estimate-hemoglobin-by-drabkin-s-solution\">Procedure to estimate Hemoglobin (by Drabkin\u2019s solution)<\/h2>\n\n\n\n<ol><li>Take 0.02ml of blood + 4 ml Drabkin\u2019s solution= 1:200 dilution.<\/li><li>Or, you can also take 0.02 ml of blood + 5 ml of Drabkin\u2019s solution= 1:250 dilution.<\/li><li>Mix the solution.<\/li><li>Within 6 hours of mixing the blood with the Drabkin\u2019s solution, take readings.<\/li><li>At filter 540, record the readings on the spectrophotometer.<\/li><li>Read against blank of Drabkin\u2019s solution.<\/li><li>Read the standard (12 G\/dL) solution with the identical dilution as that of the test sample.<\/li><li>Take readings from spectrophotometer, which are called the optical density (OD).<\/li><\/ol>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" width=\"500\" height=\"344\" src=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Figure-shows-the-results-of-hemoglobin-oxyhemoglobin-carboxyhemoglobin-and-methemoglobin-calculated-by-Drabkins-solution-1.jpg\" alt=\"Figure shows the results of hemoglobin oxyhemoglobin carboxyhemoglobin and methemoglobin calculated by Drabkins solution\" class=\"wp-image-7040\" srcset=\"https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Figure-shows-the-results-of-hemoglobin-oxyhemoglobin-carboxyhemoglobin-and-methemoglobin-calculated-by-Drabkins-solution-1.jpg 500w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Figure-shows-the-results-of-hemoglobin-oxyhemoglobin-carboxyhemoglobin-and-methemoglobin-calculated-by-Drabkins-solution-1-300x206.jpg 300w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" width=\"600\" height=\"84\" src=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Drabkins-solution-formula-for-procedure-for-hemoglobin-estimation.jpg\" alt=\"\" class=\"wp-image-7054\" srcset=\"https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Drabkins-solution-formula-for-procedure-for-hemoglobin-estimation.jpg 600w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2022\/01\/Drabkins-solution-formula-for-procedure-for-hemoglobin-estimation-300x42.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><figcaption><em>Hb (Hemoglobin) calculation formula (optical density &#8211; OD)<\/em><\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"normal-hemoglobin-level\">Normal Hemoglobin Level<\/h2>\n\n\n\n<p>Hemoglobin levels are usually the same in both male and female infants which gradually rises with the increase in age. The normal level of Hb of the respective gender and age is given in the table below.<\/p>\n\n\n\n<figure class=\"wp-block-table aligncenter\"><table><tbody><tr><td><strong>Gender<\/strong><\/td><td><strong>Age<\/strong><\/td><td><strong>Normal Hb level:<\/strong><\/td><\/tr><tr><td>Male &amp; female<\/td><td>1-5 years<\/td><td>10.9 &#8211; 15.0 g\/dl<\/td><\/tr><tr><td>Male &amp; female<\/td><td>5-11 years<\/td><td>11.9 -15.0 g\/dl<\/td><\/tr><tr><td>Male<\/td><td>Adult<\/td><td>14 &#8211; 18 g\/dl<\/td><\/tr><tr><td>Female<\/td><td>Adult<\/td><td>12 &#8211; 16 g\/dl<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"physiological-variation-of-hb\">Physiological Variation Of Hb<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"factors-causing-an-increase-in-the-level-of-hb\">Factors causing an increase in the level of Hb<\/h3>\n\n\n\n<ol><li>Respiratory and heart diseases<\/li><li>Testosterone and other hormones<\/li><li>Cigarette smoking<\/li><li>Kidney diseases<\/li><li>High altitude<\/li><li>Polycythemia<\/li><\/ol>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"factors-causing-a-decrease-in-the-level-of-hb\">Factors causing a decrease in the level of Hb<\/h3>\n\n\n\n<ol><li>Genetic disorders in the production of hemoglobin<\/li><li>Gastric Bypass Surgery<\/li><li>Iron deficiency<\/li><li>Blood loss<\/li><li>Increased levels of heavy metals and toxins in the body<\/li><li>Aging<\/li><li>Some medications<\/li><li>Pregnancy<\/li><li>Bone marrow disorder<\/li><li>Chronic kidney diseases<\/li><li>Other vitamin and mineral deficiencies<\/li><li>Disorders and diseases that disrupt the nutrient absorption<\/li><li>Abnormal disruption of RBCs<\/li><li>Hypothyroidism<\/li><li>Chronic inflammatory diseases<\/li><li>Obesity<\/li><\/ol>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"false-causes-of-raised-hemoglobin\">False Causes Of raised Hemoglobin<\/h3>\n\n\n\n<ol><li>After hemorrhage<\/li><li>Dehydration&nbsp;<\/li><li>Tested during I\/V infusion, if the I\/V contains iron<\/li><li>Burns<\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"frequently-asked-questions\">Frequently Asked Questions<\/h2>\n\n\n<div id=\"rank-math-faq\" class=\"rank-math-block\">\n<div class=\"rank-math-list \">\n<div id=\"faq-question-1642528350934\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \">Q1. What methods are used to estimate the level of hemoglobin?<\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Ans. There are many methods available by which the be level of hemoglobin can be estimated like the Sahli&#8217;s\/acid hematin method and cyanmethemoglobin method.<\/p>\n\n<\/div>\n<\/div>\n<div id=\"faq-question-1642528361191\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \">Q2. Is Drabkin\u2019s solution sensitive to light?<\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Ans. Yes, Drabkin\u2019s solution is sensitive to light. It is advised to be stored at room temperature in a place that is away from light. If it is stored in the recommended conditions then it will remain stable for 6 months.<\/p>\n\n<\/div>\n<\/div>\n<div id=\"faq-question-1642528374156\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \">Q3. What is Drabkin\u2019s reagent composed of?<\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Ans. Drabkin\u2019s reagent is composed of potassium ferricyanide, potassium cyanide, potassium hydrogen phosphate, and distal water.<\/p>\n\n<\/div>\n<\/div>\n<div id=\"faq-question-1642528405641\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \">Q4. Why is Drabkin\u2019s method more reliable than hemoglobin estimation?<\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Ans. Drabkin\u2019s method is more reliable as its readings are comparatively precise. This is because a spectrophotometer is used, so error due to subjective visual matching is eliminated.<\/p>\n\n<\/div>\n<\/div>\n<div id=\"faq-question-1642528423882\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \">Q5. Is sulfhemoglobin irreversible?<\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Ans. Yes, sulfhemoglobin is irreversible. It means that sulfhemoglobin lasts up to the lifetime of an erythrocyte.<\/p>\n\n<\/div>\n<\/div>\n<div id=\"faq-question-1642528442449\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \">Q6. Is sulfhemoglobin normal hemoglobin?<\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Ans. No, sulfhemoglobin is a rare, abnormal form of hemoglobin. It doesn\u2019t carry oxygen. The formation of sulfhemoglobin may result from particular medications such as metoclopramide, sulfonamide, dapsone, nitrates, etc.<\/p>\n\n<\/div>\n<\/div>\n<div id=\"faq-question-1642528457515\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \">Q7. \u00a0Can drinking too much water lower the level of hemoglobin?<\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Ans. Yes. The level of hemoglobin can be dropped by as much as a gram to a gram and a half, with the intake of a sufficient quantity of water.<\/p>\n\n<\/div>\n<\/div>\n<div id=\"faq-question-1642528513234\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \"><strong>Q8. Which foods lower the level of hemoglobin?<\/strong><\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Ans. Some foods lower the level of hemoglobin in your body, which are listed below<br \/>1. Eggs<br \/>2. Nuts<br \/>3. Meat<br \/>4. Whole foods<br \/>5. Cheese<\/p>\n\n<\/div>\n<\/div>\n<div id=\"faq-question-1642528594430\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \">Q9. What is the pH of Drabkin\u2019s reagent?<\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Ans. Drabkin\u2019s reagent is slightly alkaline with a pH of 8.6.<\/p>\n\n<\/div>\n<\/div>\n<div id=\"faq-question-1642529034454\" class=\"rank-math-list-item\">\n<h3 class=\"rank-math-question \">Q10. What is cyanmethemoglobin?<\/h3>\n<div class=\"rank-math-answer \">\n\n<p>Cyanmethemoglobin is a comparatively nontoxic compound of cyanide with <a href=\"https:\/\/medicine.en-academic.com\/161391\/Drabkin_solution\" rel=\"nofollow noopener\" target=\"_blank\">methemoglobin<\/a>. It is formed when methylene blue is given in cases of cyanide poisoning.<\/p>\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n<p><strong>References<\/strong><\/p>\n\n\n\n<ol><li><em>https:\/\/www.healthline.com\/health\/normal-hemoglobin-levels<\/em><\/li><li><em>https:\/\/www.sciencedirect.com\/topics\/biochemistry-genetics-and-molecular-biology\/hemoglobin-blood-level<\/em><\/li><li><em>https:\/\/labs.selfdecode.com\/blog\/symptoms-low-high-hemoglobin-diseases-increase-decrease\/<\/em><\/li><li><em>https:\/\/www.sigmaaldrich.com\/US\/en\/product\/sigma\/d5941<\/em><\/li><li><em>https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK259\/<\/em><\/li><li><em>https:\/\/medicostimes.com\/satyanarayana-biochemistry-pdf\/<\/em><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Drabkin&#8217;s solution is used to estimate the amount of hemoglobin that is present in the blood of the individual. Drabkin&#8217;s solution helps in the detection of many processes such as hemolysis in RBCs, etc. Drabkin\u2019s Solution For Hemoglobin Estimation There are kits available commercially, which makes it easy to prepare the solution. When blood passes&#8230;<\/p>\n","protected":false},"author":4,"featured_media":6985,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"rank_math_lock_modified_date":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[6],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts\/6886"}],"collection":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/comments?post=6886"}],"version-history":[{"count":0,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts\/6886\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/media\/6985"}],"wp:attachment":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/media?parent=6886"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/categories?post=6886"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/tags?post=6886"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}