{"id":687,"date":"2021-01-15T10:01:20","date_gmt":"2021-01-15T10:01:20","guid":{"rendered":"https:\/\/laboratoryinfo.com\/?p=687"},"modified":"2022-03-07T19:49:40","modified_gmt":"2022-03-07T19:49:40","slug":"wade-fite-staining-technique-for-mycobacterium-leprae","status":"publish","type":"post","link":"https:\/\/laboratoryinfo.com\/wade-fite-staining-technique-for-mycobacterium-leprae\/","title":{"rendered":"Wade-Fite Staining Technique For Mycobacterium leprae"},"content":{"rendered":"\n<p>The&nbsp;<strong>Wade-Fite<\/strong>&nbsp;staining technique is a less common special staining useful in Histopathology. It is the modification of&nbsp;<a href=\"https:\/\/laboratoryinfo.com\/zn-stain\/\" target=\"_blank\" rel=\"noreferrer noopener\">Ziehl-Neelsen Staining Method<\/a>&nbsp;to demonstrate&nbsp;<em><u>Mycobacterium leprae<\/u><\/em>&nbsp;in tissue sections, which is much less acid and alcohol fast than the tubercle bacilli.<\/p>\n\n\n<style>.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-content-wrap{padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);background-color:#ecf2f7;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-contents-title-wrap{padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-contents-title{font-weight:regular;font-style:normal;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-content-wrap .kb-table-of-content-list{font-weight:regular;font-style:normal;margin-top:var(--global-kb-spacing-sm, 1.5rem);margin-right:0px;margin-bottom:0px;margin-left:0px;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-basiccircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-basiccircle .kb-table-of-contents-icon-trigger:before, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-arrowcircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-arrowcircle .kb-table-of-contents-icon-trigger:before, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-xclosecircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-xclosecircle .kb-table-of-contents-icon-trigger:before{background-color:#ecf2f7;}<\/style>\n\n\n<h2 class=\"wp-block-heading\">Principle of&nbsp; WADE-FITE STAIN<\/h2>\n\n\n\n<p>Mycobacterium leprae in comparision to Mycobacterium tuberculosis are much less acid and alcohol fast. The leprosy bacilli&#8217;s lipid envelope is much more affected by the fat solvents (xylene) traditionally used to dewax sections.<\/p>\n\n\n\n<p>Hence, they get frequently decolorized by the standard Ziehl-Neelsen technique. Due to these factors, a modification on the standard&nbsp;<a href=\"https:\/\/laboratoryinfo.com\/zn-stain\/\" target=\"_blank\" rel=\"noreferrer noopener\">Ziehl-Neelsen staining technique<\/a>, Wade-Fite staining technique is used for the demonstration of leprosy bacilli.<\/p>\n\n\n\n<p>The Wade-Fite technique&nbsp;<strong>combines the peanut oil<\/strong>&nbsp;with the deparafinizing solvent (xylene). This minimizes the exposure of the&nbsp;<a href=\"https:\/\/laboratoryinfo.com\/various-shapes-and-arrangements-of-bacterial-cells\/\" target=\"_blank\" rel=\"noreferrer noopener\">bacterial cell<\/a>&nbsp;to organic solvents, thus protects the precarious acid-fastness of the organism.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Requrements for WADE-FITE STAIN<\/h2>\n\n\n\n<ul><li><strong>Carbol fuchsin staining solution<\/strong>&nbsp;:<br>Basic fuchsin = 1 gm<br>Absolute alcohol = 10 ml<br>5% aqueous phenol = 100 ml<br>(Dissolve basic fuchsin in absolute alcohol then add 5% aq. phenol, mix and filter before use.)<\/li><\/ul>\n\n\n\n<ul><li><strong>3% HCl in ethanol<\/strong>&nbsp;:<br>conc. HCl = 3 ml<br>Distilled water = 2 ml<br>Ethanol = 90 ml<\/li><\/ul>\n\n\n\n<ul><li><strong>Acidified&nbsp;<\/strong><a href=\"https:\/\/laboratoryinfo.com\/emb-agar-eosin-methylene-blue-agar\/\" target=\"_blank\" rel=\"noreferrer noopener\">Methylene Blue<\/a>&nbsp;:<br>Methylene Blue = 0.25 gm<br>acetic acid = 1 ml<br>Distilled water = 99 ml<\/li><\/ul>\n\n\n\n<ul><li><strong>Dewax solution<\/strong>&nbsp;:<br>Xylene : Peanut oil = 2 : 1<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Procedure of WADE-FITE STAIN<\/h2>\n\n\n\n<ol><li>Slightly warm the sections and deparaffinize in a mixture of peanut oil and xylene (1:2), two changes, 6 minutes each.<\/li><li>Blot dry and wash in running tap water for 3 minutes. Repeat if any xyline-oil remains on the section.<\/li><li>Stain with carbol fuchsin at room temperature for 20-25 minutes.<\/li><li>Wash in running tap water for 3 minutes.<\/li><li>Differentiate with 3% HCl in ethanol for 2 minutes. The sections should be pale pink on rinsing in tap water.<\/li><li>Wash in tap water for 5 minutes.<\/li><li>Counterstain in acidified methylene blue for 20-30 seconds.<\/li><li>Wash in running tap water,&nbsp;<strong>donot<\/strong>&nbsp;dehydrate in alcohol.<\/li><li>Blot sections dry and then place in 56C oven for 5 minutes.<\/li><li>Once dry, one quick dip in xylene to clear.<\/li><li>Mount with permanent mounting media.<\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\">Results and interpretation<\/h2>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-full is-resized\"><a href=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2016\/01\/wade-fite-stain.jpg\" rel=\"attachment wp-att-688\"><img decoding=\"async\" src=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2016\/01\/wade-fite-stain.jpg\" alt=\"wade-fite-stain-mycobacterium-leprae\" class=\"wp-image-688\" width=\"400\" srcset=\"https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2016\/01\/wade-fite-stain.jpg 600w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2016\/01\/wade-fite-stain-300x237.jpg 300w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2016\/01\/wade-fite-stain-20x16.jpg 20w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/a><figcaption>Tissue section showing Mycbacterium leprae (Wade-Fite stain)<\/figcaption><\/figure><\/div>\n\n\n\n<ul><li>Mycobacterium leprae : Bright Red<\/li><li>Nuclei and Background : Blue<\/li><\/ul>\n","protected":false},"excerpt":{"rendered":"<p>The&nbsp;Wade-Fite&nbsp;staining technique is a less common special staining useful in Histopathology. It is the modification of&nbsp;Ziehl-Neelsen Staining Method&nbsp;to demonstrate&nbsp;Mycobacterium leprae&nbsp;in tissue sections, which is much less acid and alcohol fast than the tubercle bacilli. Principle of&nbsp; WADE-FITE STAIN Mycobacterium leprae in comparision to Mycobacterium tuberculosis are much less acid and alcohol fast. The leprosy bacilli&#8217;s&#8230;<\/p>\n","protected":false},"author":4,"featured_media":688,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"rank_math_lock_modified_date":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[45,76,73,74,75,72],"acf":[],"_links":{"self":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts\/687"}],"collection":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/comments?post=687"}],"version-history":[{"count":0,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts\/687\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/media\/688"}],"wp:attachment":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/media?parent=687"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/categories?post=687"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/tags?post=687"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}