{"id":152,"date":"2021-01-11T18:04:41","date_gmt":"2021-01-11T18:04:41","guid":{"rendered":"https:\/\/laboratoryinfo.com\/?p=152"},"modified":"2022-03-07T19:44:16","modified_gmt":"2022-03-07T19:44:16","slug":"lactophenol-cotton-blue-lpcb","status":"publish","type":"post","link":"https:\/\/laboratoryinfo.com\/lactophenol-cotton-blue-lpcb\/","title":{"rendered":"Lactophenol Cotton Blue (LPCB): Principle and Procedure for Fungal Staining"},"content":{"rendered":"\n<p>The Lactophenol Cotton Blue (LPCB) wet mount is most widely used method for the staining and observation of fungi.<\/p>\n\n\n<style>.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-content-wrap{padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);background-color:#ecf2f7;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-contents-title-wrap{padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-contents-title{font-weight:regular;font-style:normal;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-table-of-content-wrap .kb-table-of-content-list{font-weight:regular;font-style:normal;margin-top:var(--global-kb-spacing-sm, 1.5rem);margin-right:0px;margin-bottom:0px;margin-left:0px;}.kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-basiccircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-basiccircle .kb-table-of-contents-icon-trigger:before, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-arrowcircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-arrowcircle .kb-table-of-contents-icon-trigger:before, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-xclosecircle .kb-table-of-contents-icon-trigger:after, .kb-table-of-content-nav.kb-table-of-content-id6113_3b9ebd-8c .kb-toggle-icon-style-xclosecircle .kb-table-of-contents-icon-trigger:before{background-color:#ecf2f7;}<\/style>\n\n\n<h2 class=\"wp-block-heading\">Principle<\/h2>\n\n\n\n<p>LPCB is a stain used for making semi-permanent microscopic preparation of fungi. The LPCB stain has following three components :<br><b>Phenol<\/b> : Kills any live organism.<br><b>Lactic acid<\/b> : Preserves fungal structures.<br><b>Cotton blue<\/b> : Stains the chitin and cellulose of the fungal cell wall intensely blue.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Procedure<\/h2>\n\n\n\n<ol><li>Place a drop of 70% ethanol on a clean microscopic glass slide<\/li><li>Immerse the specimen in the drop of alcohol<\/li><li>Add one or at most two drops of the LPCB before the alcohol dries out<\/li><li>Holding the coverslip between the index finger and thumb, touch one edge of the drop of mountant with a coverslip edge and lower gently avoiding air bubbles<\/li><li>This preparation is now ready for examination<\/li><li>Make the initial examination using low power objective. Switch to higher power (40X) objective for more detailed examination of spores and other structures<\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\">Result<\/h2>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-full is-resized\"><a href=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/aspergillus-3.jpg\"><img decoding=\"async\" src=\"http:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/aspergillus-3.jpg\" alt=\"lpcb-mount\" class=\"wp-image-153\" width=\"500\" srcset=\"https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/aspergillus-3.jpg 1602w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/aspergillus-3-300x203.jpg 300w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/aspergillus-3-1024x692.jpg 1024w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/aspergillus-3-768x519.jpg 768w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/aspergillus-3-1536x1037.jpg 1536w, https:\/\/laboratoryinfo.com\/wp-content\/uploads\/2015\/03\/aspergillus-3-20x14.jpg 20w\" sizes=\"(max-width: 1602px) 100vw, 1602px\" \/><\/a><figcaption>Aspergillus on LPCB Mount<\/figcaption><\/figure><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Lactophenol Cotton Blue (LPCB) wet mount is most widely used method for the staining and observation of fungi. Principle LPCB is a stain used for making semi-permanent microscopic preparation of fungi. The LPCB stain has following three components :Phenol : Kills any live organism.Lactic acid : Preserves fungal structures.Cotton blue : Stains the chitin&#8230;<\/p>\n","protected":false},"author":4,"featured_media":153,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"rank_math_lock_modified_date":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[8],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts\/152"}],"collection":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/comments?post=152"}],"version-history":[{"count":0,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/posts\/152\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/media\/153"}],"wp:attachment":[{"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/media?parent=152"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/categories?post=152"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/laboratoryinfo.com\/wp-json\/wp\/v2\/tags?post=152"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}