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Comments on: Manual Cell Counting With Neubauer Chamber https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/ All About medical Laboratories : Microbiology, Biochemistry, hematology, histopathology, virology, bacteriology, Mycology and Parasitology Tue, 12 Nov 2024 22:03:31 +0000 hourly 1 https://wordpress.org/?v=6.3.5 By: Jumecita https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-332919 Tue, 12 Nov 2024 22:03:31 +0000 https://laboratoryinfo.com/?p=871#comment-332919 Need to double check my math for a post vasectomy sample – that still has sperm, per AUA use all 9 nines squares to count.
N = number of cells (sperm)
Area = 9 mm2
Average of cells = mean of cells on both sides of the chamber / 9 squares
Dilution = usually undiluted “0”
Depth = 0.1mm

Concentration cells/ml = average of cells x dilution x 1000(where is this from) / # squares

]]> By: Jumecita https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-328323 Thu, 12 Sep 2024 14:07:20 +0000 https://laboratoryinfo.com/?p=871#comment-328323 we are going to use this to count post vasectomy sperm. Literature recommends no dilution but doesn’t mention area to count.

]]> By: Diego Méndez https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-327045 Wed, 28 Aug 2024 13:52:30 +0000 https://laboratoryinfo.com/?p=871#comment-327045 In reply to asad.

It comes from the product of area, depth and dillution.
Area = 4
Depth = 1/10
Dillution = 1/20
1/20 × 1/10 × 4 = 4/200 =1/50
Considering that you are dividing cell count by 1/50 thats is equal to multiplying cell count by 50.

]]> By: A https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-300970 Fri, 27 Oct 2023 15:48:02 +0000 https://laboratoryinfo.com/?p=871#comment-300970 The dilution factor is 20, so if you take 20/0.1 (depth factor) * 4 (squares counted) = 50 – they just shortened the math, leaving out very important steps for the beginner to follow.

]]> By: abhimanyu patra https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-8124 Mon, 25 Sep 2017 05:42:56 +0000 https://laboratoryinfo.com/?p=871#comment-8124 thanks

]]> By: Hsin-Tsung Ho https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-3996 Sat, 25 Mar 2017 01:33:39 +0000 https://laboratoryinfo.com/?p=871#comment-3996 I just purchased 3 Neubauer Chambers. One colleague who used Nageotte hemocytometer wondered my decision, since he emphysized his support from Lutz publication at Transfusion Vol 33 No 5 Pages 409-412, 1993. He mentioned Nageotte calculates WBC in 50 uL, however, Neubauer counts WBC only in 0.1 uL x 5. Sampling bias is his concern. Could you please help me solving this question? Thank you very much!

]]> By: Alkali https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-3578 Thu, 16 Feb 2017 12:36:06 +0000 https://laboratoryinfo.com/?p=871#comment-3578 I need more explanation on d preparation of d sample. some should pls help

]]> By: Mohit koli https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-3086 Fri, 16 Dec 2016 17:51:34 +0000 https://laboratoryinfo.com/?p=871#comment-3086 In reply to zara.

Let N be the no.of wbcs in all the 4 sqaures.
Volume of one wbc square =1×1×0.1=0.1mm3
Volume of 4 wbc squares =0.4mm3
0.4mm3 of wbc squares contain wbcs=N
1mm3 of wbc squares contain wbcs=N/0.4
Multiplying with dilution factor viz20 =N×20/0.4=N×50.

]]> By: Sanjeevkumar p https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-3082 Thu, 15 Dec 2016 08:45:05 +0000 https://laboratoryinfo.com/?p=871#comment-3082 Good attempt.Pls go ahead

]]> By: zara https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/#comment-3021 Wed, 07 Dec 2016 10:39:50 +0000 https://laboratoryinfo.com/?p=871#comment-3021 how to count fungal spores through this chamber ?

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