Concentration cells/ml = average of cells x dilution x 1000(where is this from) / # squares
]]>It comes from the product of area, depth and dillution.
Area = 4
Depth = 1/10
Dillution = 1/20
1/20 × 1/10 × 4 = 4/200 =1/50
Considering that you are dividing cell count by 1/50 thats is equal to multiplying cell count by 50.